| 质粒名称: | BCR/ABL P190 transgenic construct |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 38185 |
| 存储实验室: | Nora Heisterkamp |
|
载体骨架基本信息 |
|
| 载体名称: | pSK |
| 载体出品公司: | -- |
| 空载体大小: | 3000 bp |
| 完整质粒大小: | 13000 bp |
| 载体修饰: | -- |
| 载体类型: | 转基因小鼠的构建 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | BCR/ABL P190 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 10000 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | 截短型小鼠MT启动子 |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Sst II (未破坏) |
| 3’克隆位点: | Sst II (未破坏) |
| 5’测序引物 | M13-F20 |
| 3’测序引物 | M13-rev(?) |
|
文献引用方法 |
|
| 材料和方法部分: | BCR/ABL P190 transgenic construct was a gift from Nora Heisterkamp (Addgene plasmid # 38185 ; http://n2t.net/addgene:38185 ; RRID:Addgene_38185) |
| 参考文献部分: | Acute leukaemia in bcr/abl transgenic mice. Heisterkamp N, Jenster G, ten Hoeve J, Zovich D, Pattengale PK, Groffen J. Nature. 1990 Mar 15;344(6263):251-3. 10.1038/344251a0 PubMed 2179728 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS BCR/ABL P190 transgenic construct 13290 bp ds-DNA circular SYN 08-FEB-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS BCR/ABL P190 transgenic construct
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13290)
AUTHORS Heisterkamp N, Jenster G, ten Hoeve J, Zovich D, Pattengale PK,
Groffen J
TITLE Acute leukaemia in bcr/abl transgenic mice.
JOURNAL Nature. 1990 Mar 15;344(6263):251-3.
PUBMED 2179728
REFERENCE 2 (bases 1 to 13290)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 8, 2022 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13290
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(171..1031)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 794..813
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1032..1136)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1162..1617)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1299..1320)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 1511..1530
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 1744..1766
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 1758..1775
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 1759..1775
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1782..1801
/label=T7
/note="T7 promoter, forward primer"
promoter 1782..1800
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 1809..1916
/label=MCS
/note="pBluescript multiple cloning site"
primer_bind 1825..1841
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 1826..1842
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1876..1892)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(12208..12224)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(12261..12279)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(12300..12316)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(12300..12316)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(12313..12335)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 12324..12340
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(12348..12378)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 12393..12414
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(12531..12548)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(12702..13290)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(12782..12801)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
1801 gcgaattggg taccgggccc cccctcgagg tcgacggtat cgataagctt gatatcgaat
1861 tcctgcagcc cgggggatcc actagttcta gagcggccgc caccgcggtg gagctctgca
1921 ctccgcccga aaagtgcgct cggctctgcc aaggacgcgg ggcgcgtgac tatgcgtggg
1981 ctggagcaac cgcctgctgg gtgcaaaccc tttgcgcccg gactcgtcca acgactataa
2041 agagggcagg ctgtcctcta agcgtcacca cgacttcaac gtcctgagta ccttctcctc
2101 acttactccg tagctccagc ttcaccagat cccccggccg cgccatggtg gacccggtgg
2161 gcttcgcgga ggcgtggaag gcgcagttcc cggactcaga gcccccgcgc atggagctgc
2221 gctcagtggg cgacatcgag caggagctgg agcgctgcaa ggcctccatt cggcgcctgg
2281 agcaggaggt gaaccaggag cgcttccgca tgatctacct gcagacgttg ctggccaagg
2341 aaaagaagag ctatgaccgg cagcgatggg gcttccggcg cgcggcgcag gcccccgacg
2401 gcgcctccga gccccgagcg tccgcgtcgc gcccgcagcc agcgcccgcc gacggagccg
2461 acccgccgcc cgccgaggag cccgaggccc ggcccgacgg cgagggttct ccgggtaagg
2521 ccaggcccgg gaccgcccgc aggcccgggg cagccgcgtc gggggaacgg gacgaccggg
2581 gaccccccgc cagcgtggcg gcgctcaggt ccaacttcga gcggatacgc aagggccatg
2641 gccagcccgg ggcggacgcc gagaagccct tctacgtgaa cgtcgagttt caccacgagc
2701 gcggcctggt gaaggtcaac gacaaagagg tgtcggaccg catcagctcc ctgggcagcc
2761 aggccatgca gatggagcgc aaaaagtccc agcacggcgc gggctcgagc gtgggggatg
2821 catccaggcc cccttaccgg ggacgctcct cggagagcag ctgcggcgtc gacggcgact
2881 acgaggacgc cgagttgaac ccccgcttcc tgaaggacaa cctgatcgac gccaatggcg
2941 gtagcaggcc cccttggccg cccctggagt accagcccta ccagagcatc tacgtcgggg
3001 gcatgatgga aggggagggc aagggcccgc tcctgcgcag ccagagcacc tctgagcagg
3061 agaagcgcct tacctggccc cgcaggtcct actccccccg gagttttgag gattgcggag
3121 gcggctatac cccggactgc agctccaatg agaacctcac ctccagcgag gaggacttct
3181 cctctggcca gtccagccgc gtgtccccaa gccccaccac ctaccgcatg ttccgggaca
3241 aaagccgctc tccctcgcag aactcgcaac agtccttcga cagcagcagt ccccccacgc
3301 cgcagtgcca taagcggcac cggcactgcc cggttgtcgt gtccgaggcc accatcgtgg
3361 gcgtccgcaa gaccgggcag atctggccca acgatggcga gggcgccttc catggagacg
3421 caggtgagtt cctcacgcca cgtgcgtggg cacacctgca cgggggagga aaaccataga
3481 cgagtagggg atacaaaaca gaagttggga gggaggagtg gatagttttg agtgtatcag
3541 gtgcacttgt ggttcacgcg gatcctgcac ccgaacaaac tccatcccct cctccttcct
3601 gaatgcatac tgttagtgtt tggaatggaa ttgggttgag gatggtcttg tgagggatgg
3661 gaggtggttt gttcctttgg ttctagagca atgtgtttgt gaagtacagc ttgtgagttg
3721 aaggaagatg cttcaggcag taacctcctt gctatgtgct ctctccatgc cgggcagtga
3781 ggatggcata aaagccggag tcatctctag cgcccacatc ggatgctgtt agcagggact
3841 gatttatgtc ttgggcattc cccgccaccc cactgatcct gttctggagg ggttatatga
3901 cacgtttgtg aaacctgaga tatgctagct gctcctaggc catagaagtg tcaggtttgc
3961 ttgctaacca gcagtcctga acacaagtca cagcaggtga gaataggaat tagaaggaag
4021 tgaggcgaca ggcagttgtg actcagtggg ccaccgaaaa tcaactgcat ggagatttta
4081 tctgggggtg aagcagagcc acacccgttc tgatctgcct ggctttgatg ggaggcttca
4141 cccatgatta ttcaccacca ggctcctcca tggtggtaac ccagccccat ttgctgaccc
4201 tccagtgtgc tcctgctgcc tcatcttgcc aaggataagc acccacaggt gcttatttgg
4261 ggctcccaat ctgtggtcag attctagcac taagattaaa actatgggat tgtacgccta
4321 acttctgtgc tagatgcaaa taccatcagg cattttgggt catgggacta tgttttgaga
4381 tccagacaca gtttgccatt ggggtaagga aagggtgggc tggggtgtgt gtgtgaggct
4441 gtcgggaatc ttttggagtc ctggtccctg ttaagctcat ctggttttct gcttcattga
4501 tttttctttt tctgctgatg gaggagttga atgtgcttgg tttgtcaaaa tcctcttcaa
4561 acgagtgagg ctatttttgg ctcttagagc tgctgccttt ttgtggtggg cactctgtcc
4621 tgtttgtgtt aggtacttcc agttactgat cttccatttg cagctggggt ttcctgtggt
4681 gcacggctcc gacctgccgt ctggccgggc tgcattccct aagtccctat gtgggggcgg
4741 ggtggaaggc tgtactattc agaagcctgc tttgcaggca catcagctcg ttcggggtct
4801 ggaggattgc cttgtggtta ctgggcacca gccttgtctg gccagctgtt ggtcaggcga
4861 gccagcttga actggccctc agtgccatgg tgcaggagag ttattaataa agaagaaaat
4921 gagaggtttt tagggagatg tgcaaattga aattgatagt ctttgctggt ggagaattcc
4981 tgcagcccgg gggatctact tgaggttatt tgatttgctg gtttgcaaga actcgtctac
5041 acgttgtctt tatcacaaag aaattctgga gcatttgagc cctgttcaaa aaaaaaaggc
5101 aagaagaaac ttcaggaaca aataacctat ttgaccctgt aatgaataat attcacacat
5161 tttctaattt aagagctgtt gattgatttc caacttttag aatctgtttc agacaaaaca
5221 tggaagttta gtaacagttt tttctattct gtaataataa ttgccacaag gttaaaaaaa
5281 aaaatttaca gaagaacata tttgagggtg gtcgtggggg caagaaagct ggaaggacgg
5341 gtggggtgtg ttaatagcct tgtcttgtga agtaagtcct cagagacgct gtcctagttg
5401 gaagaacagg cagtgaaggt gtgcgtctgt tacggagaat aggactccag tgatctagct
5461 ggtacttaga gaggtggggg tgatcaccaa aaaaacagct ctgcctctgg gagcggagag
5521 gactgggata gaaagtattt gtctttcatt ttaagccctt ctgtacgatt tgacttttct
5581 tttctacagg cacctgccac catgcccggc tgatttttgt atttgtagta aagacaggat
5641 ttcactgtgt tggtcaggtt ggtcttgaac tcctgacctc atgatccacc cccggctttt
5701 ttttttgaca cggagtttct cgttacccag gctggagtgc agtggcgcaa tctctgctca
5761 ctgagacctc cacctcctgg gttcaagcga ttctcctgcc tcagcctcct gagtagctgg
5821 gactacaggt gtgcgtcacc atgcccggct aatttttgta tgtctaatgg agacagggtt
5881 tcaccatgtt ggtcaagttg gtctcaaact cctgaactca ggtgatccac caccttggcc
5941 tcccaaagtg ctgggattac gggcgtgagc caccatgccc ggctaaagca agaaattttc
6001 attgcatatt ttaagcaaag gcaaatgcat atgtggatag actgttttaa tttgactaaa
6061 gtcatattga atccatgaat tttagaagct caaactattg gggaacaata attaccacct
6121 tggagtgaaa atacttaatt tccacaagat ttagtaaagg aagagttttt taaaaaccac
6181 cttaatgata atagtatgta cagatgttaa gaaatgaaat aggaatgtgt aatgttggaa
6241 acacaaatat ttttgcttct gagaataaaa ctaatttttt ctcccaattt tctcttcctt
6301 tttctttttt ctgttccccc ctttctcttc cagaagccct tcagcggcca gtagcatctg
6361 actttgagcc tcagggtctg agtgaagccg ctcgttggaa ctccaaggaa aaccttctcg
6421 ctggacccag tgaaaatgac cccaaccttt tcgttgcact gtatgatttt gtggccagtg
6481 gagataacac tctaagcata actaaaggta aaagggttgt gggcagctag tggtggttgc
6541 aggagataga aatctgggaa ttgcggtttg acctaccacc ctttgctcgt taaaggagca
6601 gctttgaaat ctggactgca gggatatcca aaacaacaac tgcatgtttc taagggagtc
6661 gactctcctt agaggagttc ttgtacaata gccctgggca aaaacagaac ttgccctatt
6721 ttttatactg aaaaggacag ctggacaaaa tactgaacgc aatttttccc ctaagaaaaa
6781 gcattatttc cctaaaatgt cttatattag gaacagagca cttgaataaa cataattgat
6841 ttataaaaac tgaggctata cacttaccta tctgttcagt acaaacagga agcttcaaat
6901 gtaaacgtga attctcatac acttataaat gcatatcttt atgtggactt gttaaaatga
6961 aattggtatt taggaatttg gagattttta gtagttacac aagaatcaat gaaaaagaac
7021 gaagctggtt tccaaagctg atatgtctga tttggttcct ttcttctcag gtgaaaagct
7081 ccgggtctta ggctataatc acaatgggga atggtgtgaa gcccaaacca aaaatggcca
7141 aggctgggtc ccaagcaact acatcacgcc agtcaacagt ctggagaaac actcctggta
7201 ccatgggcct gtgtcccgca atgccgctga gtatctgctg agcagcggga tcaatggcag
7261 cttcttggtg cgtgagagtg agagcagtcc tggccagagg tccatctcgc tgagatacga
7321 agggagggtg taccattaca ggatcaacac tgcttctgat ggcaagctct acgtctcctc
7381 cgagagccgc ttcaacaccc tggccgagtt ggttcatcat cattcaacgg tggccgacgg
7441 gctcatcacc acgctccatt atccagcccc aaagcgcaac aagcccactg tctatggtgt
7501 gtcccccaac tacgacaagt gggagatgga acgcacggac atcaccatga agcacaagct
7561 gggcgggggc cagtacgggg aggtgtacga gggcgtgtgg aagaaataca gcctgacggt
7621 ggccgtgaag accttgaagg aggacaccat ggaggtggaa gagttcttga aagaagctgc
7681 agtcatgaaa gagatcaaac accctaacct ggtgcagctc cttggggtct gcacccggga
7741 gcccccgttc tatatcatca ctgagttcat gacctacggg aacctcctgg actacctgag
7801 ggagtgcaac cggcaggagg tgaacgccgt ggtgctgctg tacatggcca ctcagatctc
7861 gtcagccatg gagtacctgg agaagaaaaa cttcatccac agagatcttg ctgcccgaaa
7921 ctgcctggta ggggagaacc acttggtgaa ggtagctgat tttggcctga gcaggttgat
7981 gacaggggac acctacacag cccatgctgg agccaagttc cccatcaaat ggactgcacc
8041 cgagagcctg gcctacaaca agttctccat caagtccgac gtctgggcat ttggagtatt
8101 gctttgggaa attgctacct atggcatgtc cccttacccg ggaattgacc tgtcccaggt
8161 gtatgagctg ctagagaagg actaccgcat ggagcgccca gaaggctgcc cagagaaggt
8221 ctatgaactc atgcgagcat gttggcagtg gaatccctct gaccggccct cctttgctga
8281 aatccaccaa gcctttgaaa caatgttcca ggaatccagt atctcagacg aagtggaaaa
8341 ggagctgggg aaacaaggcg tccgtggggc tgtgagtacc ttgctgcagg ccccagagct
8401 gcccaccaag acgaggacct ccaggagagc tgcagagcac agagacacca ctgacgtgcc
8461 tgagatgcct cactccaagg gccagggaga gagcgatcct ctggaccatg agcctgccgt
8521 gtctccattg ctccctcgaa aagagcgagg tcccccggag ggcggcctga atgaagatga
8581 gcgccttctc cccaaagaca aaaagaccaa cttgttcagc gccttgatca agaagaagaa
8641 gaagacagcc ccaacccctc ccaaacgcag cagctccttc cgggagatgg acggccagcc
8701 ggagcgcaga ggggccggcg aggaagaggg ccgagacatc agcaacgggg cactggcttt
8761 cacccccttg gacacagctg acccagccaa gtccccaaag cccagcaatg gggctggggt
8821 ccccaatgga gccctccggg agtccggggg ctcaggcttc cggtctcccc acctgtggaa
8881 gaagtccagc acgctgacca gcagccgcct agccaccggc gaggaggagg gcggtggcag
8941 ctccagcaag cgcttcctgc gctcttgctc cgcctcctgc gttccccatg gggccaagga
9001 cacggagtgg aggtcagtca cgctgcctcg ggacttgcag tccacgggaa gacagtttga
9061 ctcgtccaca tttggagggc acaaaagtga gaagccggct ctgcctcgga agagggcagg
9121 ggagaacagg tctgaccagg tgacccgagg cacagtaacg cctcccccca ggctggtgaa
9181 aaagaatgag gaagctgctg atgaggtctt caaagacatc atggagtcca gcccgggctc
9241 cagcctgccc aacctgactc caaaacccct ccggcggcag gtcaccgtgg cccctgcctc
9301 gggcctcccc cacaaggaag aagctggaaa gggcagtgcc ttagggaccc ctgctgcagc
9361 tgagccagtg acccccacca gcaaagcagg ctcaggtgca ccagggggca ccagcaaggg
9421 ccccgccgag gagtccagag tgaggaggca caagcactcc tctgagtcgc cagggaggga
9481 caaggggaaa ttgtccaggc tcaaacctgc cccgccgccc ccaccagcag cctctgcagg
9541 gaaggctgga ggaaagccct cgcagagccc gagccaggag gcggccgggg aggcagtcct
9601 gggcgcaaag acaaaagcca cgagtctggt tgatgctgtg aacagtgacg ctgccaagcc
9661 cagccagccg ggagagggcc tcaaaaagcc cgtgctcccg gccactccaa agccacagtc
9721 cgccaagccg tcggggaccc ccatcagccc agcccccgtt ccctccacgt tgccatcagc
9781 atcctcggcc ctggcagggg accagccgtc ttccaccgcc ttcatccctc tcatatcaac
9841 ccgagtgtct cttcggaaaa cccgccagcc tccagagcgg atcgccagcg gcgccatcac
9901 caagggcgtg gtcctggaca gcaccgaggc gctgtgcctc gccatctcta ggaactccga
9961 gcagatggcc agccacagcg cagtgctgga ggccggcaaa aacctctaca cgttctgcgt
10021 gagctatgtg gattccatcc agcaaatgag gaacaagttt gccttccgag aggccatcaa
10081 caaactggag aataatctcc gggagcttca gatctgcccg gcgacagcag gcagtggtcc
10141 agcggccact caggacttca gcaagctcct cagttcggtg aaggaaatca gtgacatagt
10201 gcagaggtag cagcagtcag gggtcaggtg tcaggcccgt cggagctgcc tgcagcacat
10261 gcgggctcgc ccatacccgt gacagtggct gacaagggac tagtgagtca gcaccttggc
10321 ccaggagctc tgcgccaggc agagctgagg gccctgtgga gtccagctct actacctacg
10381 tttgcaccgc ctgccctccc gcaccttcct cctccccgct ccgtctctgt cctcgaattt
10441 tatctgtgga gttcctgctc cgtggactgc agtcggcatg ccaggacccg ccagccccgc
10501 tcccacctag tgccccagac tgagctctcc aggccaggtg ggaacggctg atgtggactg
10561 tctttttcat ttttttctct ctggagcccc tcctcccccg gctgggcctc cttcttccac
10621 ttctccaaga atggaagcct gaactgaggc cttgtgtgtc aggccctctg cctgcactcc
10681 ctggccttgc ccgtcgtgtg ctgaagacat gtttcaagaa ccgcatttcg ggaagggcat
10741 gcacgggcat gcacacggct ggtcactctg ccctctgctg ctgcccgggg tggggtgcac
10801 tcgccatttc ctcacgtgca ggacagctct tgatttgggt ggaaaacagg gtgctaaagc
10861 caaccagcct ttgggtcctg ggcaggtggg agctgaaaag gatcgaggca tggggcatgt
10921 cctttccatc tgtccacatc cccagagccc agctcttgct ctcttgtgac gtgcactgtg
10981 aatcctggca agaaagcttg agtctcaagg gtggcaggtc actgtcactg ccgacatccc
11041 tcccccagca gaatggaggc aggggacaag ggaggcagtg gctagtgggg tgaacagctg
11101 gtgccaaata gccccagact gggcccaggc aggtctgcaa gggcccagag tgaaccgtcc
11161 tttcacacat ctgggtgccc tgaaagggcc cttcccctcc cccactcctc taagacaaag
11221 tagattctta caaggccctt tcctttggaa caagacagcc ttcacttttc tgagttcttg
11281 aagcatttca aagccctgcc tctgtgtagc cgccctgaga gagaatagag ctgccactgg
11341 gcacctgcgc acaggtggga ggaaagggcc tggccagtcc tggtcctggc tgcactcttg
11401 aactgggcga atgtcttatt taattaccgt gagtgacata gcctcatgtt ctgtgggggt
11461 catcagggag ggttaggaaa accacaaacg gagcccctga aagcctcacg tatttcacag
11521 agcacgcctg ccatcttctc cccgaggctg ccccaggccg gagcccagat acgggggctg
11581 tgactctggg cagggacccg gggtctcctg gaccttgaca gagcagctaa ctccgagagc
11641 agtgggcagg tggccgcccc tgaggcttca cgccgggaga agccaccttc ccaccccttc
11701 ataccgcctc gtgccagcag cctcgcacag gccctagctt tacgctcatc acctaaactt
11761 gtactttatt tttctgatag aaatggtttc ctctggatcg ttttatgcgg ttcttacagc
11821 acatcacctc tttgcccccg acggctgtga cgcagccgga gggaggcact agtcaccgac
11881 agcggccttg aagacagagc aaagcgccca cccaggtccc ccgactgcct gtctccatga
11941 ggtactggtc ccttcctttt gttaacgtga tgtgccacta tattttacac gtatctcttg
12001 gtatgcatct tttatagacg ctcttttcta agtggcgtgt gcatagcgtc ctgccctgcc
12061 ccctcggggg cctgtggtgg ctccccctct gcttctcggg gtccagtgca ttttgtttct
12121 gtatatgatt ctctgtggtt ttttttgaat ccaaatctgt cctctgtagt attttttaaa
12181 taaatccgga attcctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg
12241 tggagctcca gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg
12301 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
12361 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
12421 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
12481 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
12541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
12601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
12661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
12721 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
12781 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
12841 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
12901 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
12961 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
13021 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
13081 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
13141 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
13201 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
13261 cagattacgc gcagaaaaaa aggatctcaa
//
